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    Protease Inhibitors   蛋白酶抑制劑

    單組分蛋白酶抑制劑    Single component Protease Inhibitors

    Cat.No.
    Product
    M.W.
    Description/Specifity of Inhibitor
    Solubility Stability
    ConcentrationRangea)
    Size
    AEBSF-HCl
    239.7
    Irreversible inhibitor of Thrombin and other serine proteases. Inhibits by acylation of the active site of the enzyme. Much less toxic than PMSF and DFP.
    凝血酶及其他絲氨酸蛋白酶的不可逆抑制劑,將活性位點;。比PMSF 和DFP毒性小得多。
    H2O,Aqueous solutions are stable between pH 5-6
    0.1 - 2 µM
    500 mg
    1 g
     
    Amastatin-HCl
    511
    Non-toxic reversible metallo -protease inhibitor. Inhibits many membrane-bound peptidases which are critical regulators of peptide hormones, e.g. aminopeptidase A and M, but not aminopeptidase B. Inhibits also leucine minopeptidase
    金屬蛋白酶可逆的抑制劑,無毒?梢种圃S多肽激素類的膜結合肽,如氨基肽酶A和M(但不包括氨基肽酶B),也抑制亮氨酸氨基肽酶。
    Ethanol
     0.5 % AcOH
    1 - 100 µM
    1 mg
    ε-Aminocaproic acid
    131.2
    Highly active inhibitor of fibrinolysin and chymotrypsin.
    溶纖維蛋白酶和胰凝乳蛋白酶的高活性抑制劑。
    H2O
    1 - 20 µM
    100 g
    α-Antichymot-ypsin from human plasma
    68000
    Glycoprotein that inhibits chymotrypsin-like proteases (above all human neutrophil cathepsin G) by forming stable complexes. Acute phase protein; concentration in plasma increases after events like inflammation or tissue damage
    通過形成穩定的復合物抑制胰凝乳蛋白酶類(包括所有嗜中性人組織蛋白酶G)的糖蛋白。急性時相蛋白;在發炎或組織損傷時在血漿中濃度會增加。
    Aqueous buffers
    Used at equimolar concentration
    1 mg
    Antipain-HCL
    678.2
    Reversible inhibitor of serine and cysteine proteases. Inhibits papain and trypsin more specificly than leupeptin. Plasmin is inhibited only slightly. Also involved in inhibition of RNA synthesis
    絲氨酸和半胱氨酸蛋白酶可逆的抑制劑。比亮抑酶肽抑制木瓜蛋白酶和胰蛋白酶的專一性更高。
    H2O
    Methanol DMSO
    1 - 100 µM
    5 mg
    25 mg
    Antithrombin III from human plasma
    ca. 60000
    Glycoprotein that plays a major role in controlling serine proteases in the blood clotting cascade. Inactivates above all thrombin by forming an extremely stable complex, an effect which is enhanced by heparin. Inhibits also other proteases of the coagulation cascade like plasmin, kallikrein, factor IXa, Xa, XIa & XIIa.
    主要在血液凝結中控制絲氨酸蛋白酶的糖蛋白。通過形成非常穩定的復合物抑制凝血酶的活性,可增強肝素鈉的作用。也可抑制其他凝集類的蛋白酶,如纖維蛋白溶酶、激肽釋放酶、IXa, Xa, XIa 和 XIIa因子。
    H2O
    Used at equimolar concentrations
    1 mg
    α1-Antitrypsin from human plasma α1-proteinase inhibitor
    ca.
    53000
    Glycoprotein that is mainly involved in the control of neutrophil elastase activity. Inhibits also most of other mammalian serine proteases but at a lower rate. Blocks the action of target enzymes by binding nearly irreversibly to their active site.
    主要控制嗜中性酯酶活性的糖蛋白。也抑制大多數其他哺乳動物絲氨酸蛋白酶但速率低。與附近目標酶的活性位點不可逆的結合。
    H2O
    Aqueous buffers
     
    Used at equimolar concentra tions
    5 mg
    Aprotinin (Trypsin inhibitor from bovine lung)
    ca.
    6500
    Basic single-chain polypeptide that inhibits numerous serine proteases by binding to the active site of the enzyme, form ing tight complexes. It inhibits above all plasmin, kallikrein, trypsin, chymotrypsin and urokinase but not carboxypeptidase A and B, papain, pepsin, subtilisin, thrombin and factor X. Used in cell culture to prevent proteolytic damage to cells and to extend lifetime of cells.
    基本的單鏈多肽,通過與酶的活性位點緊密結合形成復合物,抑制大多數的絲氨酸蛋白酶。抑制所有的纖維蛋白溶酶、激肽釋放酶、胰蛋白酶、胰凝乳蛋白酶和尿激酶,但不包括羧肽酶A和B、木瓜蛋白酶、胃蛋白酶、枯草菌蛋白酶、凝血酶和X因子。用于細胞培養中防止細胞蛋白的水解并延長細胞壽命。
    H2O,Aqueous buffers. Sterile iltered solutions at pH 5-8 are stable for several months. Denatures at pH > 12
    In cell culture: 0.01 - 3 µg/mL; in other applications: 10 - 250 µg/mL
    10 mg
    25 mg
    100 mg
    Arphamenine A
    387.4
    Inhibitor of the metallo-protease Aminopeptidase B
    金屬蛋白酶氨基肽酶B的抑制劑
    H2O, MeOH
    IC50:
    0.006 µg/mL
    5mg
    Arphamenine B
    403.4
    Inhibitor of the metallo-protease Aminopeptidase B
    金屬蛋白酶氨基肽酶B的抑制劑
    H2O, MeOH
    IC50:
    0.002 µg/mL
    5 mg
    Benzamidine-HCl
    174.6
    Potent inhibitor of thrombin and trypsin
    凝血酶和胰蛋白酶有效的抑制劑
    H2O
    0.1 - 50 µM
    5 g
    25 g
    Bestatin-HCl
    344.8
    Metalloprotease inhibitor with multi-pharmacological functions. Inhibits cell surface aminopeptidases (notably B) and leucine aminopeptidase. Inhibitor of leukotriene A4 hydrolase and of enkephalin degradation in cell preparations from brain. Has anticarcinogenic and immunomodulating properties.
    多種藥理學功能的金屬蛋白酶抑制劑。抑制細胞表面氨基肽酶(特別是B)和亮氨酸氨基肽酶。白三烯A4水解酶和腦細胞降解產生的腦啡肽的抑制劑。有抗癌和免疫調節功能。
    Methanol
    (5 mg/mL)
    1 - 150 µM
    Mitogenic effects at nmolar concentrations
    5 mg
    CA-074
    383.4
    Inhibitor of Cathepsin B
    組織蛋白酶B的抑制劑
    DMSO
    0.01 - 1 µM
    5 mg
    CA-074-Me
    397.5
    Proinhibitor for intracellular Cathepsin B. Membrane-permeable analog of CA-074
    細胞內組織蛋白酶B的抑制劑前體。與CA-074膜滲透性類似。
    DMSO
    1 µM
    5 mg
    Calpain Inhibitor I
    367.2
    Tripeptide aldehyde. Specific inhibitor of the Ca2+-dependent cysteine protease calpain I and of cathepsin B and L.
    三肽醛。Ca2+依賴性半胱氨酸蛋白酶鈣蛋白酶I和組織蛋白酶B 和 L專屬抑制劑
    Ethanol,
    Methanol, DMF, DMSO (10mg/mL)
    1 - 50 µM
    25 mg
    Calpain Inhibitor II
    385.2
    Tripeptide aldehyde. Specific inhibitor of the Ca2+-dependent cysteine protease calpain II and of cathepsin B and L.
    三肽醛。Ca2+依賴性半胱氨酸蛋白酶鈣蛋白酶II和組織蛋白酶B 和 L專屬抑制劑
    Ethanol, Methanol, DMF, DMSO(10 mg/mL)
    1 -50 µM
    25 mg
    Cathepsin Inhibitor Z-Phe-Gly-NHO- Bz-pMe
    489.5
    Specific inhibitor of Cathepsin B/L/S and Papain
    組織蛋白酶B/L/S和木瓜蛋白酶的專屬抑制劑
    DMSO, EtOH, Acetonitrile
    Ki:
    0.15 - 16 µM
    1 mg
    Chymostatin
    ca.
    600
    Peptide-derived aldehyde (mixture of 3 components). Reversible inhibitor of chymotrypsin-like serine and some cysteine proteases
    肽醛(含三種組分)。胰凝乳蛋白酶類絲氨酸和某些半胱氨酸蛋白酶可逆的抑制劑。
    DMSO
    Acetic acid
    10 - 100 µM
    5 mg
    25 mg
    DFP
    (Diisopropylfluoro
    phosphate)
    184.2
    A potent irreversible inhibitor of serine proteases and acetyl choline esterase. Highly toxic!
    絲氨酸蛋白酶和乙酰膽堿酯酶高效的不可逆抑制劑。劇毒!
    Isopropanol; aqueous solutions are unstable
    10 - 100 µM
    1 g
    Diprotin A
    341.5
    Reversible inhibitor of the metallo-protease Dipeptidylaminopeptidase IV
    金屬蛋白酶二肽氨基肽酶IV的可逆抑制劑
    H2O
    Ki:2.2 µM
    50 mg
    E-64
    357.4
    Non-competitive irreversible inhibitor of papain and other cysteine proteases. Forms a thioether bond with the sulfhydryl group in the active center of the enzyme. Useful for active site titration: one mole of E-64 inhibits one mole of protease.
    木瓜蛋白酶和其他半胱氨酸蛋白酶非競爭性不可逆的抑制劑。與酶活性中心的巰基形成硫醚鍵。用于活性位點的滴定:1mol的E-64抑制1mol的蛋白酶。
    H2O, DMSO Mixtures of water and ethanol
    1 - 10 µM
    5 mg
    25 mg
    E-64d (EST)
    342.4
    Membrane-permeable analog of E-64c
    與E-64c膜滲透性類似
    DMSO
    1 µM
    1 mg
    5 mg
    Ebelactone A
    338.5
    Non-toxic inhibitor for esterases, acylpeptide hydrolase, lipase and N-formylmethionine aminopeptidase.
    酯酶、;乃饷、脂肪酶和N-甲酰甲硫氨酸氨基肽酶無毒的抑制劑
    Methanol
    (200 mg/mL)
    1 - 10 µM
    1 mg
    5 mg
    Ebelactone B
    352.5
    Non-toxic inhibitor for esterase, lipase and N-formyl-methionine aminopeptidase. Inhibits also carboxypeptidase Y-like exopeptidase.
    酯酶、脂肪酶和N-甲酰甲硫氨酸氨基肽酶無毒的抑制劑。也抑制羧肽酶Y類外肽酶。
    Methanol
    (200 mg/mL)
    1 - 10 µM
    1 mg
    5 mg
    EDTA-Na2
    372.3
    Reversible inhibitor of metalloproteases
    金屬蛋白酶的可逆抑制劑
    H2O (pH 8)
    1 - 10 µM
    100 g
    1 kg
    5 kg
    EGTA
    380.4
    Inhibits metalloproteases.. Reveals high selectivity for Ca2+ over Mg2+ ions.
    Ca2+ over Mg2+ ions.
    抑制金屬蛋白酶。對Ca2+ 的選擇性高于 Mg2+。
    NH4OH, NaOH
    1 - 10 µM
    5g
    50 g
    Elastatinal
    512.6
    Inhibitor of Elastase
    彈性蛋白酶的抑制劑
    H2O, MeOH, DMSO
    Ki: 0.21 µM
    (with-Ac-Ala-Ala-Ala-OMe as substrate)
    5 mg
    Leuhistin
    241.3
    Inhibitor of Aminopeptidase M
    氨基肽酶M的抑制劑
    H2O, DMSO,
    EtOH, MeOH
    IC50:
    0.2 µg/ml
    5 mg
    Leupeptin-hemisulfate
    475.6
    Tripeptide aldehyde. Reversible competitive inhibitor of serine and cysteine proteases. Inhibits also phospholipase D and C activation in rat hepatocytes.
    三肽醛。絲氨酸和半胱氨酸蛋白酶可逆的競爭性抑制劑。也抑制鼠肝細胞中磷酸酯酶D和C的活化。
    H2O Stable for sereval months when stored in aliquots at -20 °C
    1 - 100 µM
    10 mg
    50 mg
    α2-Macroglo-bulin from human plamas
    725000
    Glycoprotein composed of 4 identical subunits. Broad-range endoproteinase inhibitor. Inhibits by forming a »trap« around the enzyme allowing only small substrate molecules to enter and to be cleaved by the entrapped protease.
    含4個相同亞單位的糖蛋白。主要的內蛋白酶抑制劑。由截留酶在酶周圍形成一個只能通過小分子底物的缺口。
    H2O Stable for several months when stored in aliquots at -20 °C
    Used at equimolar concentrations
    1 mg
     
    4-(2-Aminoethyl) -benzenesulfonyl fluoride hydrochloride PEFABLOC® SC
    239.5
    Water-soluble and relativly non-toxic irreversible inhibitor ofthrombin and other serine proteases. Inhibits by acylation of the active site of the enzyme.
    水溶性的無毒不可逆抑制劑,通過酶活性位點的;种浦颇负推渌z氨酸蛋白酶。
    H2O(20 g/100 ml) Stable for several months between pH 5 - 6; limited stability above pH 7.5
    0.1 - 5 mM in cell culture: 
    0.1 - 0.25 µM
    100 mg
    500 mg
    Pepstatin A
    685.9
    Pentapeptide derivative. Reversible inhibitor of aspartic proteases, e.g. pepsin, cathepsin D, chymosin,rennin.
    五肽的衍生物?赡娴囊种铺於彼岬鞍酌,如胃蛋白酶、組織蛋白酶D、凝乳酶
    Methanol
    (1 mg/mL) DMSO
    1 - 10 µM
    5 mg
    25 mg
    100 mg
    Phebestin
     
    441.5
    Inhibitor of Aminopeptidase N.
    氨基肽酶N的抑制劑
    H2O, DMSO, MeOH
    IC50:
    0.18 µg/ml
    1 mg
    PMSF
    Phenylmethyl sulfonyl fluoride
    174.2
    Irreversibly inhibits serine proteases by sulfonylation of the serine residue in the active site of the protease. Inhibits also papain (reversible by DTT treatment) and acetylcholin-esterase. Does not inhibit metallo-, aspartic- and most cysteine proteases.
    通過蛋白酶活性位點絲氨酸殘基的磺;,不可逆的抑制絲氨酸蛋白酶。也抑制木瓜蛋白酶(用DTT處理可逆)和乙酰膽堿酯酶。但不抑制金屬蛋白酶、天冬氨酸蛋白酶和大多數半胱氨酸蛋白酶。
    Isopropanol, ethanol, methanol. (100 - 200mM) Unstable in aqueous solution
    0.1 - 1 µM
    5 g
    25 g
    100 g
    Phosphoramidon
    587.5
    Specific inhibitor of thermolysin and neutral endopeptidase-24.11 (ANP Degradation Enzyme). Inhibits also the activity of Endothelin Converting Enzyme, collagenase and metallo-endoproteinases from many microorganisms. Does not inhibit serine, cysteine and aspartic proteases.
    嗜熱菌蛋白酶和中性內蛋白酶-24.11(ANP降解酶)的專屬抑制劑。也抑制內源性內皮素轉化酶、膠原酶和微生物來源的金屬內蛋白酶活性。
    H2O
    (20 mg/mL) DMSO Methanol
    1 - 100 µM
    5 mg
    TLCK
    (1-Chloro-3-tosylamido -7-amino-2-heptanone HCl)
    369.3
    Irreversibly inhibits trypsin but not chymotrypsin by alkylating  the histidine residue in the active site of the enzyme. Inhibits also some other serine and cysteine proteases like bromelain, ficin and papain. TLCK does not react with zymogens or in active protease-inhibitor complexes.
    通過乙;富钚晕稽c剩余的組氨酸,不可逆的抑制胰蛋白酶(但不含胰凝乳蛋白酶)。也抑制其他的絲氨酸和半胱氨酸蛋白酶,如菠蘿蛋白酶、無花果蛋白酶和木瓜蛋白酶。不與酶原或活性蛋白酶抑制劑復合物反應。
    1 mM HCl, DMSO H2O
    (20mg/mL). Aqueous solutions are unstable above pH 7
    10 - 1000 µM
    100 mg
    250 mg
    1 g
    TPCK
    (1-Chloro-3-tosylamido-4-phenyl-2-butanone)
    351.8
    Irreversibly inhibits chymotrypsin but not trypsin by specifically reacting with histidine. Inhibits also other serine and cysteine protease such as bromelain, ficin and papain.
    通過與組氨酸專一性反應,不可逆的抑制胰凝乳蛋白酶(但不含胰蛋白酶)。也抑制其他的絲氨酸和半胱氨酸蛋白酶,如菠蘿蛋白酶、無花果蛋白酶和木瓜蛋白酶。
    Ethanol (20 mg/mL) sparingly soluble in water; unstable at alkaline pH
    10 - 1000 µM
    250 mg
    1 g
    Trypsin inhibitor from egg white
    (Ovomucoid)
    ca. 28000
    Monomeric Glycoprotein. Inhibits bovine (but not human) trypsin in a 1:1 molar ratio. Inhibition is reversible and pH dependant.
    單體糖蛋白,1:1的濃度可逆的抑制牛(非人)胰蛋白酶,并與pH有關。
    H2O,1mM HCl(1mg/mL) Very stable between pH 3 - 7 against heat and 9 M urea. Unstable at alkaline pH
    Used at equimolar concentrations
    (10-100 µg/ml)
    100 mg
    Trypsin inhibitor from soybean
    ca. 22000
    Monomeric protein. Reversible serine protease inhibitor. Inhibits trypsin, factor Xa, plasmin and plasma kallikrein, but not tissue kallikrein.
    單體蛋白,絲氨酸蛋白酶的可逆抑制劑。抑制胰蛋白酶、因子Xa、血纖維蛋白溶解酶和血漿激肽釋放酶(非組織激肽釋放酶)
    H2O(1mg/mL) Sensitive to heat and high pH
    Used at equimolar concentrations (10-100 µg/ml)
    50 mg

    混合蛋白酶抑制劑    Protease Inhibitor Mixes 

    • Protease Inhibitor Mix G
    • 含5種成份的水溶性蛋白酶抑制劑,廣泛的應用于半光氨酸、絲氨酸和金屬蛋白酶的抑制,除常規使用還可用于需避免有機溶劑的環境。
    • Protease Inhibitor Mix M
    • 含6種成份,廣泛用于天冬氨酸、半光氨酸和絲氨酸蛋白酶及氨基肽酶肽酶(金屬蛋白酶)活性的抑制。特別適用于哺乳動物組織提取。
    • Protease Inhibitor Mix P
    • 含6種成份,廣泛用于天冬氨酸、半光氨酸和絲氨酸蛋白酶、氨基肽酶肽酶及金屬蛋白酶活性的抑制。特別適用于植物組織提取。
    • Protease Inhibitor Mix FY
    • 含4種成份,廣泛用于天冬氨酸、半光氨酸和絲氨酸蛋白酶金屬蛋白酶活性的抑制,特別適用于真菌及酵母提取物,可防止蛋白水解。
    • Protease Inhibitor Mix B
    • 含5種成份,廣泛用于天冬氨酸、半光氨酸和絲氨酸蛋白酶、氨基肽酶肽酶及金屬蛋白酶活性的抑制。特別適用于細菌提取物。
    • Protease Inhibitor Mix HP
    • 含4種成份的水溶性蛋白酶抑制劑,廣泛適用于半光氨酸和絲氨酸蛋白酶的抑制,不含金屬螯合物。推薦用于純化聚組氨酸標記蛋白,以及其他需避免金屬螯合劑的情況。

    所有的蛋白酶抑制劑混合物均為凍干粉末,使穩定性提高,操作更簡便,粉末可完全溶解。用1ml水或1ml 90% DMSO重新溶解后得到的溶液,足夠用于100ml的細胞溶解物或蛋白溶液的處理。溶解的蛋白酶抑制劑混合物可分裝,并在-20 °C儲存4周以上。

    Cat.No.
    Product
    Protease Inhibitor
    Acting against
    Size
    Protease Inhibitor Mix G
    (general applications ; soluble in water)
    AEBSF-HCl
    Aprotinin from boving lung
    E-64
    EDTA-Disodium
    Leupeptin
    Serine proteases
    Serine proteases
    Cysteine proteases
    Metallo proteases
    Cysteine proteases & Trypsin-like Serine proteases
    1 vial
    5 vials
    10 vials
    Protease Inhibitor Mix M
    (Mammalian cells; soluble in 90% DMSO,1 ml 90% DMSO per vial is included)
    AEBSF-HCl
    Aprotinin from boving lung
    Bestain
    E-64
    Leupeptin
    Pepstain A
    Serine proteases
    Serine proteases
    Aminopeptidase B & Leucine Aminopetidase
    Cysteine proteases
    Cysteine proteases & Trypsin-like Serine proteases
    Aspartic Protease
    1 vial
    5 vials
    10 vials
    Protease Inhibitor Mix P
    (Plant cells; soluble in 90%DMSO, 1 ml 90% DMSO per vial is included)
    AEBSF-HCl
    E-64
    Bestain
    1,10-Phenanthroline
    Pepstain A
    Leupeptin
    Serine proteases
    Cysteine proteases
    Aminopeptidase B & Leucine Aminopetidase
    Metallo proteases
    Aspartic Protease
    Cysteine proteases & Trypsin-like Serine proteases
    1 vial
    5 vials
    10 vials
    Protease Inhibitor Mix FY
    (Fungi and yeast cells; soluble in 90%DMSO, 1 ml 90% DMSO per vial is included) 
    AEBSF-HCl
    E-64
    Pepstain A
    1,10-Phenanthroline
    Serine proteases
    Cysteine proteases
    Aspartic Protease
    Metallo proteases
    1 vial
    5 vials
    10 vials
    Protease Inhibitor Mix B
    (Bacterial cells; soluble in 90%DMSO, 1 ml 90% DMSO per vial is included)
    AEBSF-HCl
    E-64
    Pepstain A
    EDTA-Disodium
    Bestain
    Serine proteases
    Cysteine proteases
    Aspartic Protease
    Metallo proteases
    Aminopeptidase B & Leucine Aminopetidase
    1 vial
    5 vials
    10 vials
    Protease Inhibitor Mix HP
    (Recombinant proteins; soluble in water)
    AEBSF-HCl
    Aprotinin from boving lung
    E-64
    Leupeptin
    Serine proteases
    Serine proteases
    Cysteine proteases
    Cysteine proteases & Trypsin-like Serine proteases
    1 vial
    5 vials
    10 vials

    Other Enzyme Inhibitors   其他酶抑制劑

    Cat.No.
    Product Name
    CAS
    Size
    Acarbose from Actinoplanes sp.  ca. 100 FIP-AIU/mg contact dried amorph
    100 mg
    500 mg
    1 g
    Chelerythrine chloride research grade
    1 mg
    5 mg
    Cyclopiazonic acid research grade
    N/A
    5 mg
    Elastase Inhibitor I
    1 mg
    Genistein research grade
    25 mg
    Hypericin research grade
    1 mg
    Levamisole.HCl analytical grade
    5 g
    1,10-Phenanthroline analytical grade
    5 g
    25 g
    Proteasome Inhibitor MG-115
    1 mg
    5 mg
    Proteasome Inhibitor MG-132
    1 mg
    5 mg
    Proteasome Inhibitor PSI
    1 mg
    5 mg
    Protein Kinase Inhibitor K-252a research grade
    0.1 mg
    Protein Kinase Inhibitor K-252b research grade
    0.1 mg
    Protein Kinase Inhibitor KT 5720 research grade
    0.1 mg
    Protein Kinase Inhibitor KT 5823 research grade
    0.1 mg
    Staurosporine research grade
    0.1 mg
    0.5 mg
    Thapsigargin research grade
    1 mg
    Wortmannin from Penicillium fumiculosum research grade
    1 mg
    5 mg
    10 mg

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